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KMID : 0903519990420030181
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1999 Volume.42 No. 3 p.181 ~ p.187
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Isolation and Antifungal Activity of the Chitinase Producing Bacterium Serratia sp. 3095 as Antagonistic Bacterium against Fusarium sp.
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Abstract
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For the selection of an effective antagonistic biocontrol agent, we have isolated an antagonistic bacterium which produced extracellular chitinase, from a local soil of Kyongju, Korea. The selected strain was identified as Serratia proteamaculans 3095. The chitinase produced from Serratia sp. 3095 showed antifungal activity which can attack the hypha surface of Fusarium oxysporum and F. solani. The carbon and nitrogen sources for chitinase production were 0.15% colloidal chitin and 0.1% ammonium sulfate, respectively. Glucose in the chitinase production medium might inhibit the production of chitinase by feed back repression. The antagonistic Serratia sp. 3095 also showed a powerful biocontrol activity against F. oxysporum through in vitro test and in vivo pot test.
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KEYWORD
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